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Abstract The spatiotemporal development of somatic tissues of the anther lobe is necessary for successful fertile pollen production. This process is mediated by many transcription factors acting through complex, multi-layered networks. Here, our analysis of functional knockout mutants of interacting basic helix–loop–helix genes Ms23, Ms32, basic helix–loop–helix 122 (bHLH122), and bHLH51 in maize (Zea mays) established that male fertility requires all four genes, expressed sequentially in the tapetum (TP). Not only do they regulate each other, but also they encode proteins that form heterodimers that act collaboratively to guide many cellular processes at specific developmental stages. MS23 is confirmed to be the master factor, as the ms23 mutant showed the earliest developmental defect, cytologically visible in the TP, with the most drastic alterations in premeiotic gene expression observed in ms23 anthers. Notably, the male-sterile ms23, ms32, and bhlh122-1 mutants lack 24-nt phased secondary small interfering RNAs (phasiRNAs) and the precursor transcripts from the corresponding 24-PHAS loci, while the bhlh51-1 mutant has wild-type levels of both precursors and small RNA products. Multiple lines of evidence suggest that 24-nt phasiRNA biogenesis primarily occurs downstream of MS23 and MS32, both of which directly activate Dcl5 and are required for most 24-PHAS transcription, with bHLH122 playing a distinct role in 24-PHAS transcription. 

Modern Information Technology (IT) servers are typically assumed to operate in quiescent conditions with almost zero static pressure differentials between inlet and exhaust. However, when operating in a data center containment system the IT equipment thermal status is a strong function of the non- homogenous environment of the air space, IT utilization workloads and the overall facility cooling system design. To implement a dynamic and interfaced cooling solution, the interdependencies of variabilities between the chassis, rack and room level must be determined. In this paper, the effect of positive as well as negative static pressure differential between inlet and outlet of servers on thermal performance, fan control schemes, the direction of air flow through the servers as well as fan energy consumption within a server is observed at the chassis level. In this study, a web server with internal air-flow paths segregated into two separate streams, each having dedicated fan/group of fans within the chassis, is operated over a range of static pressure differential across the server. Experiments were conducted to observe the steady-state temperatures of CPUs and fan power consumption. Furthermore, the server fan speed control scheme’s transient response to a typical peak in IT computational workload while operating at negative pressure differentials across the server is reported. The effects of the internal air flow paths within the chassis is studied through experimental testing and simulations for flow visualization. The results indicate that at higher positive differential pressures across the server, increasing server fans speeds will have minimal impact on the cooling of the system. On the contrary, at lower, negative differential pressure server fan power becomes strongly dependent on operating pressure differential. More importantly, it is shown that an imbalance of flow impedances in internal airflow paths and fan control logic can onset recirculation of exhaust air within the server. For accurate prediction of airflow in cases where negative pressure differential exists, this study proposes an extended fan performance curve instead of a regular fan performance curve to be applied as a fan boundary condition for Computational Fluid Dynamics simulations.